GAPDH is a typical structural protein in eukaryote organism, which plays an important role involved in the biosynthesis of cell composition and the expression of genetic information. In order to reveal the biological function of GAPDH in the growth and development of Eucalyptus, 3 genes were obtained and named as EC-GAP1, EC-GAP2 and EC-GAP3 by amplification with primers for GAPDH genes conserved regions and RACE method from the young leaf of Eucalyptus camaldulensis. The bioinformatics analysis suggested that the proteins encoded by corresponding genes owned the typical conserved domains of GAPDH and showed high homology with those of other plant species.

目的 本研究有助于了解EXP基因家族的基本特征，为深入研究其功能搭建平台。 方法 本研究对从巨桉（Eucalyptus grandis Hill）中筛选出35个EXP基因家族成员（EgrEXP1~EgrEXP35），利用生物信息学方法对其基因特征与表达模式进行综合分析。 结果 巨桉EXP基因分布在8条染色体之上，EXP蛋白均定位在细胞质膜上发挥作用，大多数的家族成员具有信号肽。巨桉EXP编码的蛋白质由α-螺旋、延伸链、无规卷曲、β-转角组成。进化分析结果表明，巨桉EXP蛋白与毛果杨（Populus trichocarpa）EXP蛋白的进化关系接近。35个巨桉EXP基因在巨桉未成熟木质部、成熟叶片、韧皮部、茎尖、木质部以及幼叶组织中表达模式存在显著差异。 结论 EXP基因家族各成员的表达模式不同，EgrEXP17、EgrEXP18可能在巨桉木材形成过程中起重要作用。